Article | 3. 2019 Vol. 3, Issue. 1
Genome-wide Association Study using the Canine SNP20 Beadchip for Canine Hip Dysplasia in Labrador Retriever



Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Wanju, 55365, South Korea1
Animal Disease & Biosecurity Team, National Institute of Animal Science, RDA, Wanju, 55365, South Korea2
Division of Animal and Dairy Science, Chungnam National University, Daejeon, Korea3




2019.3. 7:15


PDF XML




Many dog genetic disease, which can mean that the rate of inbreeding is the result. Canine hip dysplasia (CHD) is a common hereditary developmental disease of the coxofemoral joints and one of the most important bone and joint diseases. CHD is characterized by subluxation of the femoral head and deformation of the acetabulum leading to a painful osteoarthrosis. The radiographic diagnosis is sometime possible when the disease has markedly progressed. Looking up now studies with dog genetic disease a dwarfism (Germany shepherd), progressive retinal atrophy (Mastiff), malignant fever (all variety). With molecular biological techniques are being developed. This study, the hip dysplasia and early diagnosis that can be genetic markers to develop a more 22K SNP chip using the hip dysplasia in the X-ray diagnostics have Labrador retriever 48 of blood from the Wizard Genomic DNA Purification Kit using the DNA was extracted. This consists of SNP information, the entire 22,362 Canine SNP22 Beadchip used, SNP genotyping was carried out. Associated with hip phenotype for QTL detection for each SNP marker regression analysis using R statistical program (single SNP regression analysis) through the expression of hip additive effect of SNP marker for the evaluation. As the primary analysis of hip dysplasia in SNPs they have significance to the 4, 38, X chromosome respectively 11, 4, 10 results were excavated a total of 25 (genome-wise P < 0.001).



1. Baker-LePain JC, Lane NE (2010) Relationship between joint shape and the development of osteoarthritis. Curr Opin Rheumatol 22: 538-543.  

2. Budsberg SC, Lenz ME, Thonar EJ (2006) Serum and synovial fluid concentrations of keratan sulfate and hyaluronan in dogs with induced stifle joint osteoarthritis following cranial cruciate ligament transection. Am J Vet Res 67: 429-432.  

3. Dahlqvist J, Orlen H, Matsson H, Dahl N, Lonnerholm T, Gustavson KH (2009) Multiple epiphyseal dysplasia. Acta Orthop 80: 711-715.  

4. Friedenberg SG, Zhu L, Zhang Z, Foels W, Schweitzer PA, Wang W, Fisher PJ, Dykes NL, Corey E, Vernier-Singer M, Jung SW, Sheng X, Hunter LS, McDonough SP, Lust G, Bliss SP, Krotscheck U, Gunn TM, Todhunter RJ (2011) Evaluation of a fibrillin 2 gene haplotype associated with hip dysplasia and incipient osteoarthritis in dogs. Am J Vet Res 72: 530-540.  

5. Goldberg RL, Rubin AS (1989) Serum hyaluronate as a marker for disease severity in the Lactobacillus casei model of arthritis in the rat. J Rheumatol 16: 92-96.  

6. Hinrichs T, Superti-Furga A, Scheiderer WD, Bonafe L, Brenner RE, Mattes T (2010) Recessive multiple epiphyseal dysplasia (rMED) with homozygosity for C653S mutation in the DTDST gene--phenotype, molecular diagnosis and surgical treatment of habitual dislocation of multilayered patella: case report. BMC Musculoskelet Disord 11: 110.  

7. Jia J, Li L, Zhao Q, Zhang L, Ru J, Liu X, Li Q, Shi L (2012) Association of a single nucleotide polymorphism in pregnancy-associated plasma protein-A2 with developmental dysplasia of the hip: a case-control study. Osteoarthritis Cartilage 20: 60-63.  

8. Lee SH, Park BH, Sharma A, Dang CG, Lee SS, Choi TJ, Choy YH, Kim HC, Jeon KJ, Kim SD, Yeon SH, Park SB, Kang HS. 2014. Hanwoo cattle : orgin, domestication, breeding, strategies and genomic selection J. Anim. Sci. & Technol., 56: 2.  

9. Kannu P, Irving M, Aftimos S, Savarirayan R (2011) Two novel COL2A1 mutations associated with a Legg-Calve-Perthes disease-like presentation. Clin Orthop Relat Res 469: 1785-1790.  

10. Kim OH, Park H, Seong MW, Cho TJ, Nishimura G, Superti-Furga A, Unger S, Ikegawa S, Choi IH, Song HR, Kim HW, Yoo WJ, Shim JS, Chung CY, Oh CW, Jeong C, Song KS, Seo SG, Cho SI, Yeo IK, Kim SY, Park S, Park SS (2011) Revisit of multiple epiphyseal dysplasia: ethnic difference in genotypes and comparison of radiographic features linked to the COMP and MATN3 genes. Am J Med Genet A 155A: 2669-2680.  

11. Kolundzic R, Trkulja V, Mikolaucic M, Kolundzic MJ, Pavelic SK, Pavelic K (2011) Association of interleukin-6 and transforming growth factor-beta1 gene polymorphisms with developmental hip dysplasia and severe adult hip osteoarthritis: a preliminary study. Cytokine 54: 125-128.  

12. Marschall Y, Distl O (2007) Mapping quantitative trait loci for canine hip dysplasia in German Shepherd dogs. Mamm Genome 18: 861-870.  

13. Nakashima E, Kitoh H, Maeda K, Haga N, Kosaki R, Mabuchi A, Nishimura G, Ohashi H, Ikegawa S (2005) Novel COL9A3 mutation in a family with multiple epiphyseal dysplasia. Am J Med Genet A 132A: 181-184.  

14. Nganvongpanit K, Itthiarbha A, Ong-Chai S, Kongtawelert P (2008) Evaluation of serum chondroitin sulfate and hyaluronan: biomarkers for osteoarthritis in canine hip dysplasia. J Vet Sci 9: 317-325.  

15. Quignon P, Herbin L, Cadieu E, Kirkness EF, Hedan B, Mosher DS, Galibert F, Andre C, Ostrander EA, Hitte C (2007) Canine population structure: assessment and impact of intra-breed stratification on SNP-based association studies. PLoS One 2: e1324.  

16. Ray A, Shakya A, Ray BK (2005) Inflammation-responsive transcription factors SAF-1 and c-Jun/c-Fos promote canine MMP-1 gene expression. Biochim Biophys Acta 1732: 53-61.  

17. Roy DR (2011) Spectrum of intra-articular findings of the acute and subacute painful hip with multiple epiphyseal dysplasia/spondyloepiphyseal dysplasia. J Pediatr Orthop B 20: 284-286.  

18. Shi D, Dai J, Zhu P, Qin J, Zhu L, Zhu H, Zhao B, Qiu X, Xu Z, Chen D, Yi L, Ikegawa S, Jiang Q (2011) Association of the D repeat polymorphism in the ASPN gene with developmental dysplasia of the hip: a case-control study in Han Chinese. Arthritis Res Ther 13: R27  

19. Zhou Z, Sheng X, Zhang Z, Zhao K, Zhu L, Guo G, Friedenberg SG, Hunter LS, Vandenberg-Foels WS, Hornbuckle WE, Krotscheck U, Corey E, Moise NS, Dykes NL, Li J, Xu S, Du L, Wang Y, Sandler J, Acland GM, Lust G, Todhunter RJ (2010) Differential genetic regulation of canine hip dysplasia and osteoarthritis. PLoS One 5: e13219.