Article | 3. 2019 Vol. 3, Issue. 1
Detection of polymorphisms of growth differentiation factor 9 (GDF9) gene in Indonesian goats



Indonesian Goat Research Center Sei Putih, North Sumatera 20585, Indonesia1
Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.2




2019.3. 37:44


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A member of growth factor and a member of the transforming growth factor β superfamily, growth differentiation factor 9 (GDF9) has a critical role in prolificacy of sheep and goat. In this study, we analyzed exon 1 of the GDF9 gene in Indonesian goats. For this purpose, 77 blood samples representing nine Indonesian goat breeds (Gembrong, Senduro, Ettawa Grade, Boerawa, Boerka, Kosta, Samosir, Boer, and Kacang) were collected. An exonic region of GDF9 gene, 462 bp in length, was amplified using specific primers. Two single nucleotide polymorphisms (SNPs); SNP g.306G>A and SNP g.228C>A, were identified based on reference and direct sequencing, respectively. SNP g.306G>A was used to genotype all animals using RFLP method with HhaI restriction enzyme. The results revealed an absence of polymorphism of GDF9 gene at SNP g.306G>A in all animals investigated. Further study on SNP g. 228C>A showed polymorphism of GDF9 gene in all Indonesia goats except Senduro, Ettawa Grade, Boerawa and Samosir goats. In average, the frequency of C allele (88%) is higher than A allele (12%), followed by CC (77%) and CA (23%) genotypes. However, in Boer goat, the CA genotype (75%) is higher than CC genotype (25%). These population was not deviated from Hardy Weinberg Equilibrium (P>0.05) based on SNP g.228C>A. In conclusion, SNP g.228C>A might be suggested as a tool for Indonesian goat marker selection based on the gene encoding prolificacy trait.



1. FAOSTAT. 2006. CD-ROM Food and Agriculture Organization of the United Nations. Rome. Italy  

2. Yadav A and Yadav BR. 2008. DNA Fingerprint: Genetic relationship in six Indian goat breeds. Indian J. Biotechnol 7: 487-490.   

3. Elvin JA, Clark AT, Wang P, Wolfman NM and Matzuk MM. 1999. Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol. Endocrinol 13: 1035-1048.  

4. McNatty KP, Juengel JL, Reader KL, Lun S, Myllymaa S, Lawrence SB, Western A, Meerasahib MF, Mottershead DG, Groome NP, Ritvos O and Laitinen MP. 2005. Bone morphogenetic protein 15 and growth differentiation factor 9 co-operate to regulate granulosa cell function in ruminants. Reproduction 129: 481-487  

5. Sadighi M, Bodensteiner KJ, Beattie AE and Galloway SM. 2002. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5. Anim. Genet 33:244-245  

6. McNatty KP, Juengel JL, Wilson T, Galloway SM, Davis GH, Hudson NL, Moeller CL, Cranfield M, Reader KL, Laitinen MP, Groome NP, Sawyer HR and Ritvos O. 2003. Oocyte-derived growth factors and ovulation rate in sheep. Reprod. Suppl 61: 339-351.  

7. Feng T, Geng CX, Lang XZ, Chu MX, Cao GL, Di R, Fang L, Chen HQ, Liu XL and Li N. 2011. Polymorphism of caprine GDF9 gene and their association with litter size in Jining Grey goats. Mol Biol Rep 38:5189-5197.  

8. An XP, Hou JX, Zhao HB, Li G, Bai L, Peng JY, Yan QM, Song YX, Wang JG and Cao BY. 2012. Polymorphism identification in goat GNRH1 and GDF9 genes and their association analysis with litter size. Anim. Genet 44: 234-8.  

9. Silva BDM, Castro EA, Souza CJH, Paiva SR, Sartori R, Franco MM, Azevedo HC, Silva TASN, Vieira AMC, Neves JP and Melo EO. 2010. A new polymorphism in the growth and differentiation factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Anim. Genet 42:89-92.  

10. Hanrahan PJ, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R and Galloway SM. 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol. Reprod 70:900-909.  

11. Javanmard A, Azadzadeh N and Esmailizadeh AK. 2011. Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet. Res. Commun 35: 157-67.  

12. Davis GH. 2004. Fecundity genes in sheep. Anim Reprod Sci 92:87-96.  

13. Ran X, Lin J, Du Z, Qing C and Wang J. 2009. Diversity of BMP15 and GDF9 genes in White goat of Guizhou Province and evolution of the encoded proteins. Zool. Res 30:593-602  

14. Ma X, Liu X, Zhang C and Li J. 2010. Candidate genes polymorphism and its association to prolificacy in Chinese goats. J. Agric. Sci 2: 88-92.  

15. Dutta R, Das B, Laskar S, Kalita DJ, Borah P, Zaman G and Saikia DP. 2013. Polymorphism, sequencing and phylogenetic characterization of growth differentiation factor 9 (GDF9) gene in Assam Hill goat. Afr. J. Biotechnol 12: 6894-6900.  

16. Gazooei YM, Niazi A and Zamiri MJ. 2013. Single nucleotide polymorphism analysis of the bone morphogenetic protein receptor IB and growth and differentiation factor 9 genes in Rayini goats (Capra hircus). Journal of Livestock Science and Technologies 1: 45-50.   

17. Li B X, Chu MX and Wang JY. 2003. PCR-SSCP analysis on growth differentiation factor 9 gene in sheep. Acta Genet Sin 304:307-310..  

18. Ahlawat S, Sharma R, Roy M, Mandakmale S, Prakash V and Tantia MS. 2016. Genotyping of novel SNPs in BMPR1B, BMP15, and GDF9 genes for association with prolificacy in seven Indian goat breeds. Anim Biotech 27:199-207.  

19. Yan QM, Yang S, Zhu GQ, An XP, Wang JG, Song YX, and Cao BY. 2010. Polymorphism of GDF9 gene and its relation ship with litter size and growth weight of Xing Shaaneng Dairy goat and Boer goat. Chin. J. Vet. Sci 30:1394-1397.  

20. Ahlawat S, Sharma R, and Maitra A. 2013. Screening of indigenous goats for prolificacy associated DNA markers of sheep. Gene 517:128-131.  

21. Polley S, Sachinandan D, Subhasis B, Ramakant K, Paras Y, Jaspreet SA, Saibal C, Subhransu P, Biswajit B, Tirtha KD and Surender LG. 2009. Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Rumin Res 85:122-129.  

22. Ahlawat S, Sharma, R, Roy M, Tantia MS and Prakash V. 2015. Association analysis of novel SNPs in BMPR1B, BMP15 and GDF9 genes with reproductive traits in Black Bengal goats. Small Rumin Res 132:92-98.  

23. Chu MX, Wu ZH, Feng T, Cao GL, Fang L, Di R, Huang DW, Li XW and Li N. 2011. Polymophism of GDF9 gene and its association with litter size in goats. Vet Res Commun 35:329-336.  

24. Wang X, Yang Q, Wang K, Yan H, Pan C, Chen H, Liu J, Zhu H, Qu L and Lan X. 2018. Two strongly linked single nucleotide polymorphisms (Q320P and V3971) in GDF9 gene are associated with litter size in Cahsmere goats. Theriogenology 125:115-121